S100A11 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

S100A11 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A3F1

Cat no : 60024-1-PBS

Synonyms

Calgizzarin, MLN 70, MLN70, Protein S100 A11, Protein S100 C, S100A11, S100C

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Validation Data Gallery

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  WB analysis of T-47D using 60024-1-Ig (same clone as 60024-1-PBS)

  T-47D cells were subjected to SDS PAGE followed by western blot with 60024-1-Ig (S100A11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60024-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 60024-1-Ig (same clone as 60024-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 60024-1-Ig (S100A11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60024-1-PBS in a different storage buffer formulation.

• 

  WB analysis of BxPC-3 using 60024-1-Ig (same clone as 60024-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 60024-1-Ig (S100A11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60024-1-PBS in a different storage buffer formulation.

• 

  WB analysis of DU 145 using 60024-1-Ig (same clone as 60024-1-PBS)

  DU 145 cells were subjected to SDS PAGE followed by western blot with 60024-1-Ig (S100A11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60024-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 60024-1-Ig (same clone as 60024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60024-1-Ig (S100A11 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60024-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60024-1-Ig (same clone as 60024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60024-1-Ig (S100A11 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60024-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 60024-1-Ig (same clone as 60024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60024-1-Ig (S100A11 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60024-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 60024-1-Ig (same clone as 60024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60024-1-Ig (S100A11 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60024-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 60024-1-Ig (same clone as 60024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60024-1-Ig (S100A11 antibody) at dilution of 1:1000 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 60024-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 60024-1-Ig (same clone as 60024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60024-1-Ig (S100A11 antibody) at dilution of 1:1000 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 60024-1-PBS in a different storage buffer formulation.

• 

  IF Staining of BxPC-3 using 60024-1-Ig (same clone as 60024-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed BxPC-3 cells using S100A11 antibody (60024-1-Ig, Clone: 1A3F1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60024-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60024-1-PBS targets S100A11 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: S100A11 fusion protein Ag0343
• Full Name: S100 calcium binding protein A11
• Calculated Molecular Weight: 105 aa, 12 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC001410
• Gene Symbol: S100A11
• Gene ID (NCBI): 6282
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

S100A11, also named as MLN 70, Calgizzarin and S100C, belongs to the S-100 family. It facilitates the differentiation and the cornification of keratinocytes. The naming of S100A11 systematically arises from its membership of the S100 protein family, named after their solubility in 100% saturated ammonium sulfate solution. First discovered in 1989, S100A11 has since been proposed to have direct biological functions in an assortment of physiological processes such as endo- and exocytosis, regulation of enzyme activity, cell growth and regulation, apoptosis and inflammation.