S100A11 Monoclonal antibody

• Featured Product
• KD/KO Validated

S100A11 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

6

CloneNo.

1A3F1

Cat no : 60024-1-Ig

Synonyms

Calgizzarin, MLN 70, MLN70, Protein S100 A11, Protein S100 C, S100A11, S100C

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Validation Data Gallery

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  WB analysis of T-47D using 60024-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 60024-1-Ig (S100A11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 60024-1-Ig

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 60024-1-Ig (S100A11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 60024-1-Ig

  DU 145 cells were subjected to SDS PAGE followed by western blot with 60024-1-Ig (S100A11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 60024-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 60024-1-Ig (S100A11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 60024-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60024-1-Ig (S100A11 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 60024-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60024-1-Ig (S100A11 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 60024-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60024-1-Ig (S100A11 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 60024-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60024-1-Ig (S100A11 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 60024-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60024-1-Ig (S100A11 antibody) at dilution of 1:1000 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of human thyroid cancer using 60024-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60024-1-Ig (S100A11 antibody) at dilution of 1:1000 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IF Staining of BxPC-3 using 60024-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed BxPC-3 cells using S100A11 antibody (60024-1-Ig, Clone: 1A3F1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: T-47D cells, A431 cells, BxPC-3 cells, DU 145 cells
• Positive IHC detected in: human lung cancer tissue, human ovary tumor tissue, human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochemistry (IHC): IHC : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

60024-1-Ig targets S100A11 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: S100A11 fusion protein Ag0343
• Full Name: S100 calcium binding protein A11
• Calculated Molecular Weight: 105 aa, 12 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC001410
• Gene Symbol: S100A11
• Gene ID (NCBI): 6282
• RRID: AB_2238821
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S100A11, also named as MLN 70, Calgizzarin and S100C, belongs to the S-100 family. It facilitates the differentiation and the cornification of keratinocytes. The naming of S100A11 systematically arises from its membership of the S100 protein family, named after their solubility in 100% saturated ammonium sulfate solution. First discovered in 1989, S100A11 has since been proposed to have direct biological functions in an assortment of physiological processes such as endo- and exocytosis, regulation of enzyme activity, cell growth and regulation, apoptosis and inflammation.

Publications

Species	Application	Title
human		Mol Cell Proteomics; S100A11 mediates hypoxia-induced mitogenic factor (HIMF)-induced smooth muscle cell migration, vesicular exocytosis, and nuclear activation.; Authors - Fan Chunling C ; KD Validated
human	WB	Talanta; Effective enrichment of cholangiocarcinoma secretomes using the hollow fiber bioreactor culture system.; Authors - Weeraphan Churat C
human	WB	Mol Cells; Proteomic analysis of the triglyceride-rich lipoprotein-laden foam cells.; Authors - Lu Yanjun Y
human	IF	Nat Commun; Annexin A1 is a polarity cue that directs mitotic spindle orientation during mammalian epithelial morphogenesis; Authors - Maria Fankhaenel
mouse	WB	Phytomedicine; Differences in p38-STAT3-S100A11 signaling after the administration of aristolochic acid I and IVa may account for the disparity in their nephrotoxicity; Authors - Zhong Xian
mouse	WB,IHC	Cell Rep; Extracellular-vesicle-packaged S100A11 from osteosarcoma cells mediates lung premetastatic niche formation by recruiting gMDSCs; Authors - Chuangzhong Deng ; KD Validated