CoraLite® Plus 488-conjugated Rootletin Polyclonal antibody

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  IF Staining of hTERT-RPE1 using CL488-55485

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite® Plus 488 Rootletin antibody (CL488-55485) at dilution of 1:200, CoraLite®594 ARL13B antibody (CL594-17711, red).

Rootletin Polyclonal Antibody for IF/ICC

Cat No. CL488-55485

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

CROCC, KIAA0445, ROLT, Rootletin

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Tested Applications

• Positive IF/ICC detected in: hTERT-RPE1 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-55485 targets Rootletin in IF/ICC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ciliary rootlet coiled-coil, rootletin
• Calculated Molecular Weight: 229 kDa
• Observed Molecular Weight: 228 kDa
• GenBank Accession Number: NM_014675
• Gene Symbol: Rootletin
• Gene ID (NCBI): 9696
• RRID: AB_3672859
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen Affinity Purified
• UNIPROT ID: Q5TZA2
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Rootletin is predicted to enable kinesin binding activity and structural molecule activity. Involved in several processes, including centriole-centriole cohesion; positive regulation of cilium assembly; and positive regulation of protein localization to cilium.