RXRB Recombinant antibody

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  WB analysis using 84656-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84656-4-RR (RXRB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 84656-4-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RXRB antibody (84656-4-RR, Clone: 242178D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HeLa using 84656-4-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RXRB Recombinant antibody (84656-4-RR, Clone:242178D4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84656-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84656-4-RR against Human RXRB were performed. The affinity constant is 1.24 nM.

RXRB Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 84656-4-RR

Clone No.242178D4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

Retinoic acid receptor RXR-beta, RCoR 1, NR2B2, H 2RIIBP, DAUDI6

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Tested Applications

• Positive WB detected in: SK-BR-3 cells, K-562 cells
• Positive IF/ICC detected in: MCF-7 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84656-4-RR targets RXRB in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RXRB fusion protein Ag0263
• Full Name: retinoid X receptor, beta
• Calculated Molecular Weight: 57 kDa
• GenBank Accession Number: BC001167
• Gene Symbol: RXRB
• Gene ID (NCBI): 6257
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P28702
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Retinoid X Receptor beta (RXRB) is a member of the retinoid X receptor (RXR) family of nuclear receptors which are involved in mediating the effects of retinoic acid. RXRB heterodimerizes with other nuclear hormone receptors, such as RAR, the thyroid hormone receptor, and the vitamin D3 receptor, to enhance binding to their cognate response elements. (PMID: 8257090)