RXRA Recombinant antibody, PBS Only

RXRA Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240878A9

Cat no : 83796-5-PBS

Synonyms

ORF-3, RXRα, Retinoic acid receptor RXR-alpha, ORF3, NR2B1

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Validation Data Gallery

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  WB analysis using 83796-5-RR (same clone as 83796-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83796-5-RR (RXRA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83796-5-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 83796-5-RR (same clone as 83796-5-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug RXRA Recombinant antibody (83796-5-RR, Clone:240878A9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83796-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83796-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83796-5-RR against Human RXRA were performed. The affinity constant is below 1 pM.

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Product Information

The immunogen of 83796-5-PBS is RXRA Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RXRA fusion protein Ag15651
• Full Name: retinoid X receptor, alpha
• Calculated Molecular Weight: 462 aa, 51 kDa
• Observed Molecular Weight: 54 kDa, 44 kDa
• GenBank Accession Number: BC110998
• Gene Symbol: RXRA
• Gene ID (NCBI): 6256
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Retinoid X receptor alpha (RXRA). Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high-affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of a ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation, and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes. This antibody is a rabbit polyclonal antibody raised against the 350 AA of human RXRA C-terminal. RXRA is highly expressed in the liver and also expressed in the lungs, kidneys, and heart. It can recognize the mature 54 kDa RXRA and the truncated 44 kDa RXRA (PMID: 20541701).