RXRA Monoclonal antibody

RXRA Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

4H6C4

Cat no : 60198-1-Ig

Synonyms

NR2B1, Retinoid X receptor alpha, retinoid X receptor, alpha, RXRA, RXRα

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Validation Data Gallery

• 

  WB analysis of HeLa using 60198-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60198-1-Ig (RXRA antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 60198-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60198-1-Ig (RXRA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 60198-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 60198-1-Ig (RXRA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 60198-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60198-1-Ig (RXRA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 60198-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 60198-1-Ig (RXRA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 60198-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 60198-1-Ig (RXRA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 60198-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 60198-1-Ig (RXRA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Hela cells
• Positive IHC detected in: human stomach cancer tissue, mouse cerebellum tissue, rat liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

60198-1-Ig targets RXRA in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RXRA fusion protein Ag0987
• Full Name: retinoid X receptor, alpha
• Calculated Molecular Weight: 462 aa, 51 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC007925
• Gene Symbol: RXRA
• Gene ID (NCBI): 6256
• RRID: AB_10896497
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Retinoid X receptor alpha (RXRA). Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high-affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of a ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation, and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes. This antibody is a rabbit polyclonal antibody raised against the 350 AA of human RXRA C-terminal. RXRA is highly expressed in the liver, and also expressed in the lungs, kidneys, and heart. It can recognize the the mature 54 kDa RXRA and the truncated 44 kD RXRA (PMID: 20541701).

Publications

Species	Application	Title
human	WB	Int J Endocrinol; Constitutive Androstane Receptor-Mediated Inhibition of Metformin on Phase II Metabolic Enzyme SULT2A1; Authors - Xiaowen Hu