RUVBL2 Monoclonal antibody, PBS Only

RUVBL2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A1A4

Cat no : 67851-1-PBS

Synonyms

48 kDa TBP interacting protein, CGI 46, ECP 51, ECP51, INO80 complex subunit J, INO80J, Repressing pontin 52, REPTIN, Reptin 52, RuvB like 2, RuvB like 2 (E. coli), RUVBL2, RVB2, TAP54 beta, TIH2, TIP48, TIP49B

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Validation Data Gallery

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  WB analysis using 67851-1-Ig (same clone as 67851-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 67851-1-Ig (RUVBL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67851-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67851-1-Ig (same clone as 67851-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67851-1-Ig (RUVBL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67851-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67851-1-Ig (same clone as 67851-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67851-1-Ig (RUVBL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67851-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67851-1-Ig (same clone as 67851-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RUVBL2 antibody (67851-1-Ig, Clone: 1A1A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67851-1-PBS in a different storage buffer formulation.

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Product Information

67851-1-PBS targets RUVBL2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: RUVBL2 fusion protein Ag0253
• Full Name: RuvB-like 2 (E. coli)
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC000428
• Gene Symbol: RUVBL2
• Gene ID (NCBI): 10856
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

RUVBL2 belongs to the conserved ATPases associated with various cellular activities (AAA+) protein subfamily, which is characterized by the presence of conserved Walker A and B motifs that are involved in ATP binding and hydrolysis. The AAA+ (ATPases associated with diverse cellular activities) ATPases, RUVBL1 and RUVBL2, were originally isolated as components of transcriptional complexes and shown to function in a number of different cellular processes, including transcriptional regulation, chromatin remodelling and DNA damage responses. (PMID: 31572066, PMID: 31138842)