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  • Featured Product
  • KD/KO Validated

RSF1 Polyclonal antibody, PBS Only

RSF1 Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 26011-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RSF1 Polyclonal antibody (26011-1-AP)

    WB analysis using 26011-1-AP (same clone as 26011-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26011-1-AP (RSF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26011-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using RSF1 Polyclonal antibody (26011-1-AP)

    WB analysis of HeLa using 26011-1-AP (same clone as 26011-1-PBS)

    WB result of RSF1 antibody (26011-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-RSF1 transfected HeLa cells. This data was developed using the same antibody clone with 26011-1-PBS in a different storage buffer formulation.

Product Information

26011-1-PBS targets RSF1 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RSF1 fusion protein Ag22456
Full Name remodeling and spacing factor 1
Calculated Molecular Weight 1441 aa, 164 kDa
Observed Molecular Weight250 kDa
GenBank Accession NumberBC153210
Gene Symbol RSF1
Gene ID (NCBI) 51773
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RSF1, remodelling and spacing factor 1, is an important interphase centromere protein and is overexpressed in many types of cancers and correlated with poor overall survival. RSF1 has functions mainly in maintaining chromosome stability, facilitating DNA repair, maintaining the protein homeostasis of RSF1 and suppressing the transcription of some oncogenes when RSF1 protein is expressed at an optimal level; however, RSF1 overexpression facilitates drug resistance and cell cycle checkpoint inhibition to prompt cancer proliferation and survival. The RSF1 expression level and gene background are crucial for RSF1 functions, which may explain why RSF1 has different functions in different cancer types. The calculated molecular weight of RSF1 is 163 kDa, but 26011-1-AP can detect a band around 250 kDa, we validated the specificity by knock down. (PMID: 34147108)