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  • Featured Product
  • KD/KO Validated

RSF1 Polyclonal antibody

RSF1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26011-1-AP

Synonyms

HBV pX associated protein 8, HBXAP, p325, RSF 1, RSF1, XAP8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RSF1 Polyclonal antibody (26011-1-AP)

    WB analysis using 26011-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26011-1-AP (RSF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RSF1 Polyclonal antibody (26011-1-AP)

    WB analysis of HeLa using 26011-1-AP

    WB result of RSF1 antibody (26011-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-RSF1 transfected HeLa cells.

Tested Applications

Positive WB detected inCOLO 320 cells, HeLa cells, HEK-293 cells, U-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26011-1-AP targets RSF1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RSF1 fusion protein Ag22456
Full Name remodeling and spacing factor 1
Calculated Molecular Weight 1441 aa, 164 kDa
Observed Molecular Weight250 kDa
GenBank Accession NumberBC153210
Gene Symbol RSF1
Gene ID (NCBI) 51773
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RSF1, remodelling and spacing factor 1, is an important interphase centromere protein and is overexpressed in many types of cancers and correlated with poor overall survival. RSF1 has functions mainly in maintaining chromosome stability, facilitating DNA repair, maintaining the protein homeostasis of RSF1 and suppressing the transcription of some oncogenes when RSF1 protein is expressed at an optimal level; however, RSF1 overexpression facilitates drug resistance and cell cycle checkpoint inhibition to prompt cancer proliferation and survival. The RSF1 expression level and gene background are crucial for RSF1 functions, which may explain why RSF1 has different functions in different cancer types. The calculated molecular weight of RSF1 is 163 kDa, but 26011-1-AP can detect a band around 250 kDa, we validated the specificity by knock down. (PMID: 34147108)

Protocols

Product Specific Protocols
WB protocol for RSF1 antibody 26011-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Hengbin (Verified Customer) (12-05-2022)

We have generated KO cells and would be happy to test the antibody.

  • Applications: Western Blot, ChIP
  • Cell Tissue Type: cell or tissues