CoraLite® Plus 488-conjugated RRBP1 Recombinant antibody

RRBP1 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230159G12

Cat no : CL488-82890-2

Synonyms

Ribosome-binding protein 1, ES/130-related protein, ES/130 related protein, 230159G12, 180 kDa ribosome receptor homolog

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-82890-2

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 RRBP1 antibody (CL488-82890-2, Clone: 230159G12 ) at dilution of 1:200.

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  FC experiment of BxPC-3 using CL488-82890-2

  1x10^6 BxPC-3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated RRBP1 Recombinant antibody (CL488-82890-2, Clone:230159G12)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: BxPC-3 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82890-2 targets RRBP1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RRBP1 fusion protein Ag16648
• Full Name: ribosome binding protein 1 homolog 180kDa (dog)
• Calculated Molecular Weight: 1410 aa, 152 kDa
• Observed Molecular Weight: 108 kDa, 152 kDa
• GenBank Accession Number: BC128577
• Gene Symbol: RRBP1
• Gene ID (NCBI): 6238
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Ribosome-binding protein 1 (RRBP1) is an endoplasmic reticulum membrane protein. It is a 1410 amino acid protein composed of a hydrophobic NH2-terminus that includes a transmembrane domain, a highly conserved tandem repeat (ribosome-binding domain), and an acidic coiled-coil COOH-terminal domain. RRBP1 acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. It is also involved in early cardiac morphogenesis. (PMID: 22709790; 8812507; 7686155)