RRBP1 Recombinant antibody, PBS Only

RRBP1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230159G10

Cat no : 82890-3-PBS

Synonyms

Ribosome-binding protein 1, ES/130-related protein, ES/130 related protein, 230159G10, 180 kDa ribosome receptor homolog

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Validation Data Gallery

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  WB analysis using 82890-3-RR (same clone as 82890-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82890-3-RR (RRBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82890-3-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 82890-3-RR (same clone as 82890-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RRBP1 antibody (82890-3-RR, Clone: 230159G10 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82890-3-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 82890-3-RR (same clone as 82890-3-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RRBP1 Recombinant antibody (82890-3-RR, Clone:230159G10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82890-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82890-3-RR

  Biolayer interferometry (BLl) kinetic assays of 82890-3-RR against Human RRBP1 were performed. The affinity constant is 3.26 nM.

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Product Information

The immunogen of 82890-3-PBS is RRBP1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RRBP1 fusion protein Ag16648
• Full Name: ribosome binding protein 1 homolog 180kDa (dog)
• Calculated Molecular Weight: 1410 aa, 152 kDa
• GenBank Accession Number: BC128577
• Gene Symbol: RRBP1
• Gene ID (NCBI): 6238
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.