RPS6KA1 Polyclonal antibody

RPS6KA1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29224-1-AP

Synonyms

MAPKAPK 1a, MAPKAP kinase 1a, MAPK-activated protein kinase 1a, MAP kinase-activated protein kinase 1a, HU 1

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Validation Data Gallery

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  WB analysis using 29224-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29224-1-AP (RPS6KA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: K-562 cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29224-1-AP targets RPS6KA1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ribosomal protein S6 kinase, 90kDa, polypeptide 1
• Calculated Molecular Weight: 735 aa, 83 kDa
• Observed Molecular Weight: 75-95 kDa
• GenBank Accession Number: BC014966
• Gene Symbol: RPS6KA1
• Gene ID (NCBI): 6195
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases and play an important role in the MAPK signaling cascade and act downstream of ERK1/2 signaling. It can mediate mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RSK1 (RPS6KA1) is also designated as p90RSK associated with cellular proliferation, survival and differentiation.