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  • KD/KO Validated

RPS3A Polyclonal antibody

RPS3A Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 14123-1-AP

Synonyms

40S ribosomal protein S3a, Fte 1, FTE1, MFTL, ribosomal protein S3A, RPS3A



Tested Applications

Positive WB detected inHeLa cells, A431 cells, A549 cells, HepG2 cells, Jurkat cells, Raji cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14123-1-AP targets RPS3A in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, Xenopus
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPS3A fusion protein Ag5273
Full Name ribosomal protein S3A
Calculated molecular weight 30 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC066926
Gene symbol RPS3A
Gene ID (NCBI) 6189
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPS3a, a component of the ribosomal small subunit (40S), is a member of the A20 family of the deubiquitinating cysteine proteases. RPS3a is an inducing factor for oncogenic cellular transformation of Rat-1 cells by v-fos, and is highly expressed in most tumors including hepatocellular carcinoma and other cancers. On the other hand, RPS3a has also been described as a regulator of apoptosis and transcription factors

Protocols

Product Specific Protocols
WB protocol for RPS3A antibody 14123-1-APDownload protocol
IHC protocol for RPS3A antibody 14123-1-APDownload protocol
IF protocol for RPS3A antibody 14123-1-APDownload protocol
IP protocol for RPS3A antibody 14123-1-APDownload protocol
FC protocol for RPS3A antibody 14123-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
XenopusIF

Cell

Late Endosomes Act as mRNA Translation Platforms and Sustain Mitochondria in Axons.

Authors - Jean-Michel Cioni
IF

Cell Rep

On-Site Ribosome Remodeling by Locally Synthesized Ribosomal Proteins in Axons.

Authors - Christine E. Holt
human,mouseWB,IHC,IF

Cell Discov

RPS3A positively regulates the mitochondrial function of human periaortic adipose tissue and is associated with coronary artery diseases.

Authors - Yan Tang
  • KD Validated
humanWB

Mol Cell Proteomics

Proteomic landscape of exosomes reveals the functional contributions of CD151 in triple-negative breast cancer.

Authors - Sipeng Li
humanWB

Mol Cell Proteomics

Proteomic Profiling Identified Multiple Short-lived Members of the Central Proteome as the Direct Targets of the Addicted oncogenes in Cancer Cells.

Authors - Qi Tong-Gang TG
XenopusWB

Neuron

Axon-Axon Interactions Regulate Topographic Optic Tract Sorting via CYFIP2-Dependent WAVE Complex Function.

Authors - Jean-Michel Cioni