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RPS3A Polyclonal antibody
RPS3A Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Cat no : 14123-1-AP
Validation Data Gallery
|Positive WB detected in||HeLa cells, A431 cells, A549 cells, HepG2 cells, Jurkat cells, Raji cells|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human pancreas cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14123-1-AP targets RPS3A in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, Xenopus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RPS3A fusion protein Ag5273|
|Full Name||ribosomal protein S3A|
|Calculated molecular weight||30 kDa|
|Observed molecular weight||35 kDa|
|GenBank accession number||BC066926|
|Gene ID (NCBI)||6189|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
RPS3a, a component of the ribosomal small subunit (40S), is a member of the A20 family of the deubiquitinating cysteine proteases. RPS3a is an inducing factor for oncogenic cellular transformation of Rat-1 cells by v-fos, and is highly expressed in most tumors including hepatocellular carcinoma and other cancers. On the other hand, RPS3a has also been described as a regulator of apoptosis and transcription factors
|Product Specific Protocols|
|WB protocol for RPS3A antibody 14123-1-AP||Download protocol|
|IHC protocol for RPS3A antibody 14123-1-AP||Download protocol|
|IF protocol for RPS3A antibody 14123-1-AP||Download protocol|
|IP protocol for RPS3A antibody 14123-1-AP||Download protocol|
|FC protocol for RPS3A antibody 14123-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Late Endosomes Act as mRNA Translation Platforms and Sustain Mitochondria in Axons.
On-Site Ribosome Remodeling by Locally Synthesized Ribosomal Proteins in Axons.
RPS3A positively regulates the mitochondrial function of human periaortic adipose tissue and is associated with coronary artery diseases.
Mol Cell Proteomics
Proteomic landscape of exosomes reveals the functional contributions of CD151 in triple-negative breast cancer.
Mol Cell Proteomics
Proteomic Profiling Identified Multiple Short-lived Members of the Central Proteome as the Direct Targets of the Addicted oncogenes in Cancer Cells.
Axon-Axon Interactions Regulate Topographic Optic Tract Sorting via CYFIP2-Dependent WAVE Complex Function.