RPS3 Monoclonal antibody

• Featured Product
• KD/KO Validated

RPS3 Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ChIP, ELISA

Conjugate

Unconjugated

Publications

12

CloneNo.

2G7H4

Cat no : 66046-1-Ig

Synonyms

40S ribosomal protein S3, Cell and organelle markers, ribosomal protein S3, Ribosome Marker, RPS3

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Validation Data Gallery

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  WB analysis using 66046-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66046-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 66046-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 66046-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of ROS1728 using 66046-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 66046-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 66046-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 66046-1-Ig

  IP result of anti-RPS3 (IP:66046-1-Ig, 3ug; Detection:66046-1-Ig 1:2000) with HEK-293 cells lysate 1800ug.

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  IHC staining of human ovary tumor using 66046-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66046-1-Ig (RPS3 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 66046-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66046-1-Ig (RPS3 Antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66046-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 66046-1-Ig(RPS3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 66046-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 66046-1-Ig(RPS3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 66046-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas using 66046-1-Ig(RPS3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 66046-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas using 66046-1-Ig(RPS3 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 66046-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66046-1-Ig(RPS3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HSC-T6 cells, HeLa cells, human brain tissue, Neuro-2a cells, RAW 264.7 cells, ROS1728 cells, rat brain tissue, HEK-293 cells, HepG2 cells, K-562 cells, Jurkat cells, NIH/3T3 cells, 4T1 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human ovary tumor tissue, human colon cancer tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• FC: See 1 publications below
• ChIP: See 1 publications below

Product Information

66046-1-Ig targets RPS3 in WB, IP, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RPS3 fusion protein Ag7515
• Full Name: ribosomal protein S3
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC003137
• Gene Symbol: RPS3
• Gene ID (NCBI): 6188
• RRID: AB_11182493
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival. The calculated molecular weight of RPS3 is 26 kDa, but the modified RPS3 is about 33 kDa.

Publications

Species	Application	Title
human	FC	Transl Res; Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.; Authors - Mahmoud Dabbah
human	IHC	Proc Natl Acad Sci U S A; Local translation in nuclear condensate amyloid bodies.; Authors - Phaedra R Theodoridis
human	WB	PLoS Pathog; Separate domains of G3BP promote efficient clustering of alphavirus replication complexes and recruitment of the translation initiation machinery.; Authors - Benjamin Götte
human	WB	Free Radic Biol Med; Identification of interacting partners of Human Mpv17-like protein with a mitigating effect of mitochondrial dysfunction through mtDNA damage.; Authors - Reiko Iida
human	WB	Oncotarget; Ribosomal protein S3 (rpS3) secreted from various cancer cells is N-linked glycosylated.; Authors - Yong Joong Kim ; KD Validated
human	WB,IF	Front Immunol; Interactome of the Autoimmune Risk Protein ANKRD55.; Authors - Nerea Ugidos