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  • KD/KO Validated

RPS3 Monoclonal antibody

RPS3 Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2G7H4

Cat no : 66046-1-Ig

Synonyms

40S ribosomal protein S3, ribosomal protein S3, Ribosome Marker, RPS3



Tested Applications

Positive WB detected inHSC-T6 cells, human brain tissue, Neuro-2a cells, RAW 264.7 cells, ROS1728 cells, rat brain tissue, HeLa cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman ovary tumor tissue, human colon cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66046-1-Ig is RPS3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RPS3 fusion protein Ag7515
Full Name ribosomal protein S3
Calculated molecular weight 27 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC003137
Gene symbol RPS3
Gene ID (NCBI) 6188
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival. The calculated molecular weight of RPS3 is 26 kDa, but the modified RPS3 is about 33 kDa.

Protocols

Product Specific Protocols
WB protocol for RPS3 antibody 66046-1-IgDownload protocol
IHC protocol for RPS3 antibody 66046-1-IgDownload protocol
IF protocol for RPS3 antibody 66046-1-IgDownload protocol
IP protocol for RPS3 antibody 66046-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanFC

Transl Res

Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.

Authors - Mahmoud Dabbah
humanIHC

Cancer Lett

Ribosomal protein S3 regulates GLI2-mediated osteosarcoma invasion.

Authors - Hiroko Nagao-Kitamoto
mouseWB

iScience

Cdh1-APC Regulates Protein Synthesis and Stress Granules in Neurons through an FMRP-Dependent Mechanism.

Authors - Arielle N Valdez-Sinon
humanWB,IF

Front Immunol

Interactome of the Autoimmune Risk Protein ANKRD55.

Authors - Nerea Ugidos
humanWB

Oncotarget

Ribosomal protein S3 (rpS3) secreted from various cancer cells is N-linked glycosylated.

Authors - Yong Joong Kim
  • KD Validated
humanWB

Free Radic Biol Med

Identification of interacting partners of Human Mpv17-like protein with a mitigating effect of mitochondrial dysfunction through mtDNA damage.

Authors - Reiko Iida