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RPS3 Monoclonal antibody
RPS3 Monoclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 66046-1-Ig
Validation Data Gallery
|Positive WB detected in||HSC-T6 cells, human brain tissue, Neuro-2a cells, RAW 264.7 cells, ROS1728 cells, rat brain tissue, HeLa cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human ovary tumor tissue, human colon cancer tissue, human pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:20000-1:100000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 66046-1-Ig is RPS3 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||RPS3 fusion protein Ag7515|
|Full Name||ribosomal protein S3|
|Calculated molecular weight||27 kDa|
|Observed molecular weight||33 kDa|
|GenBank accession number||BC003137|
|Gene ID (NCBI)||6188|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival. The calculated molecular weight of RPS3 is 26 kDa, but the modified RPS3 is about 33 kDa.
|Product Specific Protocols|
|WB protocol for RPS3 antibody 66046-1-Ig||Download protocol|
|IHC protocol for RPS3 antibody 66046-1-Ig||Download protocol|
|IF protocol for RPS3 antibody 66046-1-Ig||Download protocol|
|IP protocol for RPS3 antibody 66046-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.
Ribosomal protein S3 regulates GLI2-mediated osteosarcoma invasion.
Cdh1-APC Regulates Protein Synthesis and Stress Granules in Neurons through an FMRP-Dependent Mechanism.
Interactome of the Autoimmune Risk Protein ANKRD55.
Ribosomal protein S3 (rpS3) secreted from various cancer cells is N-linked glycosylated.
Free Radic Biol Med
Identification of interacting partners of Human Mpv17-like protein with a mitigating effect of mitochondrial dysfunction through mtDNA damage.