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  • KD/KO Validated

RPS3 Monoclonal antibody

RPS3 Monoclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a


human, mouse, rat







Cat no : 66046-1-Ig


40S ribosomal protein S3, Cell and organelle markers, ribosomal protein S3, Ribosome Marker, RPS3

Tested Applications

Positive WB detected inHSC-T6 cells, HeLa cells, human brain tissue, Neuro-2a cells, RAW 264.7 cells, ROS1728 cells, rat brain tissue, HEK-293 cells, HepG2 cells, K-562 cells, Jurkat cells, NIH/3T3 cells, 4T1 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman ovary tumor tissue, human colon cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66046-1-Ig targets RPS3 in WB, IP, IF, FC, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RPS3 fusion protein Ag7515
Full Name ribosomal protein S3
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC003137
Gene Symbol RPS3
Gene ID (NCBI) 6188
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival. The calculated molecular weight of RPS3 is 26 kDa, but the modified RPS3 is about 33 kDa.


Product Specific Protocols
WB protocol for RPS3 antibody 66046-1-IgDownload protocol
IHC protocol for RPS3 antibody 66046-1-IgDownload protocol
IF protocol for RPS3 antibody 66046-1-IgDownload protocol
IP protocol for RPS3 antibody 66046-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Transl Res

Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.

Authors - Mahmoud Dabbah

Proc Natl Acad Sci U S A

Local translation in nuclear condensate amyloid bodies.

Authors - Phaedra R Theodoridis

PLoS Pathog

Separate domains of G3BP promote efficient clustering of alphavirus replication complexes and recruitment of the translation initiation machinery.

Authors - Benjamin Götte

Free Radic Biol Med

Identification of interacting partners of Human Mpv17-like protein with a mitigating effect of mitochondrial dysfunction through mtDNA damage.

Authors - Reiko Iida


Ribosomal protein S3 (rpS3) secreted from various cancer cells is N-linked glycosylated.

Authors - Yong Joong Kim
  • KD Validated

Front Immunol

Interactome of the Autoimmune Risk Protein ANKRD55.

Authors - Nerea Ugidos