RPS3 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

RPS3 Monoclonal Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G7H4

Cat no : 66046-1-PBS

Synonyms

40S ribosomal protein S3, Cell and organelle markers, ribosomal protein S3, Ribosome Marker, RPS3

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Validation Data Gallery

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  WB analysis using 66046-1-Ig (same clone as 66046-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66046-1-PBS in a different storage buffer formulation.

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  WB analysis using 66046-1-Ig (same clone as 66046-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66046-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 66046-1-Ig (same clone as 66046-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66046-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 66046-1-Ig (same clone as 66046-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66046-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66046-1-Ig (same clone as 66046-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66046-1-PBS in a different storage buffer formulation.

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  WB analysis of ROS1728 using 66046-1-Ig (same clone as 66046-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66046-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 66046-1-Ig (same clone as 66046-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66046-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 66046-1-Ig (same clone as 66046-1-PBS)

  IP result of anti-RPS3 (IP:66046-1-Ig, 3ug; Detection:66046-1-Ig 1:2000) with HEK-293 cells lysate 1800ug. This data was developed using the same antibody clone with 66046-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66046-1-Ig (same clone as 66046-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66046-1-Ig (RPS3 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66046-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66046-1-Ig (same clone as 66046-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66046-1-Ig (RPS3 Antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66046-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66046-1-Ig (same clone as 66046-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 66046-1-Ig(RPS3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66046-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66046-1-Ig (same clone as 66046-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 66046-1-Ig(RPS3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66046-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 66046-1-Ig (same clone as 66046-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 66046-1-Ig(RPS3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66046-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 66046-1-Ig (same clone as 66046-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 66046-1-Ig(RPS3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66046-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66046-1-Ig (same clone as 66046-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66046-1-Ig(RPS3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66046-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66046-1-PBS targets RPS3 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RPS3 fusion protein Ag7515
• Full Name: ribosomal protein S3
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC003137
• Gene Symbol: RPS3
• Gene ID (NCBI): 6188
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival. The calculated molecular weight of RPS3 is 26 kDa, but the modified RPS3 is about 33 kDa.