RPS20 Monoclonal antibody, PBS Only (Detector)

RPS20 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E9B4

Cat no : 68270-1-PBS

Synonyms

Small ribosomal subunit protein uS10, ribosomal protein S20, 40S ribosomal protein S20, 3E9B4

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Validation Data Gallery

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  WB analysis using 68270-1-Ig (same clone as 68270-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68270-1-Ig (RPS20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68270-1-PBS in a different storage buffer formulation.

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  WB analysis using 68270-1-Ig (same clone as 68270-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68270-1-Ig (RPS20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68270-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 68270-1-Ig (same clone as 68270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68270-1-Ig (RPS20 antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 68270-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68270-1-Ig (same clone as 68270-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RPS20 antibody (68270-1-Ig, Clone: 3E9B4 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68270-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50774-1

  Cytometric bead array standard curve of MP50774-1, RPS20 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68270-2-PBS. Detection antibody: 68270-1-PBS. Standard:Ag8275. Range: 1.563-200 ng/mL.

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  Cytometric bead array standard curve of MP50774-2

  Cytometric bead array standard curve of MP50774-2, RPS20 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68270-3-PBS. Detection antibody: 68270-1-PBS. Standard:Ag8275. Range: 3.125-200 ng/mL.

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Product Information

68270-1-PBS targets RPS20 as part of a matched antibody pair:

MP50774-1: 68270-2-PBS capture and 68270-1-PBS detection (validated in Cytometric bead array)

MP50774-2: 68270-3-PBS capture and 68270-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RPS20 fusion protein Ag8275
• Full Name: ribosomal protein S20
• Calculated Molecular Weight: 13 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC007507
• Gene Symbol: RPS20
• Gene ID (NCBI): 6224
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS20 is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm.