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CoraLite® Plus 488-conjugated RPS18 Recombinant antibody

RPS18 Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240004C10

Cat no : CL488-83327

Synonyms

Ke-3, KE3, KE 3, HKE3, D6S218E

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated RPS18 Recombinant an (CL488-83327)

    IF Staining of HepG2 using CL488-83327

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 RPS18 antibody (CL488-83327, Clone: 240004C10 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated RPS18 Recombinant an (CL488-83327)

    FC experiment of HepG2 using CL488-83327

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Rps18 Recombinant Antibody (CL488-83327, Clone:240004C10)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-83327 is RPS18 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RPS18 fusion protein Ag12505
Full Name ribosomal protein S18
Calculated Molecular Weight 152 aa, 18 kDa
GenBank Accession NumberBC106063
Gene Symbol RPS18
Gene ID (NCBI) 6222
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RPS18 antibody CL488-83327Download protocol
FC protocol for CL Plus 488 RPS18 antibody CL488-83327Download protocol
Standard Protocols
Click here to view our Standard Protocols