CoraLite® Plus 488-conjugated RPS12 Monoclonal antibody

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  IF Staining of HepG2 using CL488-67683

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 RPS12 antibody (CL488-67683, Clone: 1A3D1 ) at dilution of 1:200.

RPS12 Monoclonal Antibody for IF/ICC

Cat No. CL488-67683

Clone No.1A3D1

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC

Synonyms

40S ribosomal protein S12, ribosomal protein S12, RPS12

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67683 targets RPS12 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RPS12 fusion protein Ag9649
• Full Name: ribosomal protein S12
• Calculated Molecular Weight: 132 aa, 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC017321
• Gene Symbol: RPS12
• Gene ID (NCBI): 6206
• RRID: AB_2919518
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P25398
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RPS12 (ribosomal protein S12) is a plastid ribosomal protein which is a part of the 30S ribosomal subunit. Together with S4 and S5 plays an important role in translational accuracy.