RPL7 Polyclonal antibody, PBS Only

RPL7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14583-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HEK-293 using 14583-1-AP (same clone as 14583-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14583-1-AP (RPL7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14583-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 14583-1-AP (same clone as 14583-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14583-1-AP (RPL7 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14583-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 14583-1-AP (same clone as 14583-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14583-1-AP (RPL7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14583-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 14583-1-AP (same clone as 14583-1-PBS)

  IP result of anti-RPL7 (IP:14583-1-AP, 3ug; Detection:14583-1-AP 1:1000) with HEK-293 cells lysate 880ug. This data was developed using the same antibody clone with 14583-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 14583-1-AP (same clone as 14583-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 14583-1-AP (RPL7 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14583-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 14583-1-AP (same clone as 14583-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 14583-1-AP (RPL7 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14583-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 14583-1-AP (same clone as 14583-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 14583-1-AP (RPL7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14583-1-PBS in a different storage buffer formulation.

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Product Information

14583-1-PBS targets RPL7 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPL7 fusion protein Ag6104
• Full Name: ribosomal protein L7
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 29-30 kDa
• GenBank Accession Number: BC006095
• Gene Symbol: RPL7
• Gene ID (NCBI): 6129
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. RPL7 binds to G-rich structures in 28S rRNA, and plays a regulatory role in the translation apparatus, inhibits cell-free translation of mRNAs. In addition, RPL7 is suggested to have a role as an integral part of natural freeze tolerance[PMID: 18706984]. Besides, RPL7 is responsible for its normal ribosome functions including the nuclear targeting, ribosome assembly and translation[PMID:17258209].