RPL7 Polyclonal antibody

RPL7 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14583-1-AP

Synonyms

60S ribosomal protein L7, humL7 1, ribosomal protein L7, RPL7



Tested Applications

Positive WB detected inHEK-293 cells, mouse kidney tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14583-1-AP targets RPL7 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL7 fusion protein Ag6104
Full Name ribosomal protein L7
Calculated molecular weight 29 kDa
Observed molecular weight 29-30 kDa
GenBank accession numberBC006095
Gene symbol RPL7
Gene ID (NCBI) 6129
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. RPL7 binds to G-rich structures in 28S rRNA, and plays a regulatory role in the translation apparatus, inhibits cell-free translation of mRNAs. In addition, RPL7 is suggested to have a role as an integral part of natural freeze tolerance[PMID: 18706984]. Besides, RPL7 is responsible for its normal ribosome functions including the nuclear targeting, ribosome assembly and translation[PMID:17258209].

Protocols

Product Specific Protocols
WB protocol for RPL7 antibody 14583-1-APDownload protocol
IHC protocol for RPL7 antibody 14583-1-APDownload protocol
IF protocol for RPL7 antibody 14583-1-APDownload protocol
IP protocol for RPL7 antibody 14583-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Nat Cell Biol

METTL16 exerts an m6A-independent function to facilitate translation and tumorigenesis.

Authors - Rui Su
humanIHC

Proc Natl Acad Sci U S A

Local translation in nuclear condensate amyloid bodies.

Authors - Phaedra R Theodoridis
humanWB,RIP

Biochem Biophys Res Commun

CRISPR-based RNA-binding protein mapping in live cells.

Authors - Baiwen Chen
humanWB

Front Endocrinol (Lausanne)

Glucose controls co-translation of structurally related mRNAs via the mTOR and eIF2 pathways in human pancreatic beta cells

Authors - Manuel Bulfoni
humanWB

Science

Endosomal lipid signaling reshapes the endoplasmic reticulum to control mitochondrial function

Authors - Wonyul Jang