RPL7 Polyclonal antibody

RPL7 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 14583-1-AP


60S ribosomal protein L7, humL7 1, ribosomal protein L7, RPL7

Tested Applications

Positive WB detected inHEK-293 cells, mouse kidney tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14583-1-AP targets RPL7 in WB, IP, IF, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL7 fusion protein Ag6104
Full Name ribosomal protein L7
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 29-30 kDa
GenBank Accession NumberBC006095
Gene Symbol RPL7
Gene ID (NCBI) 6129
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. RPL7 binds to G-rich structures in 28S rRNA, and plays a regulatory role in the translation apparatus, inhibits cell-free translation of mRNAs. In addition, RPL7 is suggested to have a role as an integral part of natural freeze tolerance[PMID: 18706984]. Besides, RPL7 is responsible for its normal ribosome functions including the nuclear targeting, ribosome assembly and translation[PMID:17258209].


Product Specific Protocols
WB protocol for RPL7 antibody 14583-1-APDownload protocol
IHC protocol for RPL7 antibody 14583-1-APDownload protocol
IF protocol for RPL7 antibody 14583-1-APDownload protocol
IP protocol for RPL7 antibody 14583-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Hematol Oncol

METTL16 promotes liver cancer stem cell self-renewal via controlling ribosome biogenesis and mRNA translation

Authors - Meilin Xue

Nat Cell Biol

METTL16 exerts an m6A-independent function to facilitate translation and tumorigenesis.

Authors - Rui Su

Proc Natl Acad Sci U S A

Local translation in nuclear condensate amyloid bodies.

Authors - Phaedra R Theodoridis

Biochem Biophys Res Commun

CRISPR-based RNA-binding protein mapping in live cells.

Authors - Baiwen Chen

Front Endocrinol (Lausanne)

Glucose controls co-translation of structurally related mRNAs via the mTOR and eIF2 pathways in human pancreatic beta cells

Authors - Manuel Bulfoni


Endosomal lipid signaling reshapes the endoplasmic reticulum to control mitochondrial function

Authors - Wonyul Jang