RPL3 Monoclonal antibody, PBS Only

RPL3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G1B8

Cat no : 66130-1-PBS

Synonyms

60S ribosomal protein L3, ribosomal protein L3, RPL3, TARBP B

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Validation Data Gallery

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  WB analysis of RAW 264.7 using 66130-1-Ig (same clone as 66130-1-PBS)

  Western blot analysis of RPL3 in various cell lines using Proteintech antibody 66130-1-Ig at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66130-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66130-1-Ig (same clone as 66130-1-PBS)

  Western blot analysis of RPL3 in various cell lines and tissues using Proteintech antibody 66130-1-Ig at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66130-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66130-1-Ig (same clone as 66130-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66130-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66130-1-Ig (same clone as 66130-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66130-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66130-1-Ig (same clone as 66130-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66130-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66130-1-Ig (same clone as 66130-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66130-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66130-1-Ig (same clone as 66130-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66130-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66130-1-Ig (same clone as 66130-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66130-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66130-1-Ig (same clone as 66130-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66130-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66130-1-Ig (same clone as 66130-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66130-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66130-1-Ig (same clone as 66130-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66130-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66130-1-Ig (same clone as 66130-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66130-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66130-1-Ig (same clone as 66130-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66130-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66130-1-Ig (same clone as 66130-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66130-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66130-1-Ig (same clone as 66130-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL3 antibody (66130-1-Ig, Clone: 2G1B8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66130-1-PBS in a different storage buffer formulation.

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Product Information

66130-1-PBS targets RPL3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RPL3 fusion protein Ag20400
• Full Name: ribosomal protein L3
• Calculated Molecular Weight: 46/27 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC012786
• Gene Symbol: RPL3
• Gene ID (NCBI): 6122
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL3, also named as TARBP-B and MGC104284, belongs to the ribosomal protein L3P family. This protein is a component of the 60S subunit. It can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.