RPL17 Monoclonal antibody, PBS Only

RPL17 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C5E6

Cat no : 67223-1-PBS

Synonyms

60S ribosomal protein L17, 60S ribosomal protein L23, FLJ92089, PD 1, ribosomal protein L17, RPL17, rpL23

---

Validation Data Gallery

• 

  WB analysis using 67223-1-Ig (same clone as 67223-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67223-1-Ig (RPL17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67223-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 67223-1-Ig (same clone as 67223-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67223-1-Ig (RPL17 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67223-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 67223-1-Ig (same clone as 67223-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67223-1-Ig (RPL17 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67223-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67223-1-Ig (same clone as 67223-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL17 antibody (67223-1-Ig, Clone: 1C5E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67223-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67223-1-Ig (same clone as 67223-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67223-1-Ig (RPL17 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67223-1-PBS in a different storage buffer formulation.

---

Product Information

67223-1-PBS targets RPL17 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RPL17 fusion protein Ag5393
• Full Name: ribosomal protein L17
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 20-23 kDa
• GenBank Accession Number: BC066323
• Gene Symbol: RPL17
• Gene ID (NCBI): 6139
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL17 gene encodes a ribosomal protein that is a component of the 60S subunit belonging to the L22P family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as rpL23 because the encoded protein shares amino acid identity with ribosomal protein L23 from Halobacterium marismortui; however, its official symbol is RPL17.