RPL17 Monoclonal antibody

RPL17 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

1C5E6

Cat no : 67223-1-Ig

Synonyms

60S ribosomal protein L17, 60S ribosomal protein L23, FLJ92089, PD 1, ribosomal protein L17, RPL17, rpL23

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Validation Data Gallery

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  WB analysis using 67223-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67223-1-Ig (RPL17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 67223-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67223-1-Ig (RPL17 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 67223-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67223-1-Ig (RPL17 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67223-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL17 antibody (67223-1-Ig, Clone: 1C5E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  IF Staining of HepG2 using 67223-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67223-1-Ig (RPL17 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

67223-1-Ig targets RPL17 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RPL17 fusion protein Ag5393
• Full Name: ribosomal protein L17
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 20-23 kDa
• GenBank Accession Number: BC066323
• Gene Symbol: RPL17
• Gene ID (NCBI): 6139
• RRID: AB_2882514
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL17 gene encodes a ribosomal protein that is a component of the 60S subunit belonging to the L22P family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as rpL23 because the encoded protein shares amino acid identity with ribosomal protein L23 from Halobacterium marismortui; however, its official symbol is RPL17.

Publications

Species	Application	Title
rat	WB,IHC	J Orthop Translat; Comparative proteomic analysis identifies differentially expressed proteins and reveals potential mechanisms of traumatic heterotopic ossification progression.; Authors - Zhenyuan Wei
human	WB	iScience; TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment; Authors - Magdalena Wolczyk
human	WB,IF	J Virol; Involvement of ribosomal protein L17 and Y-box binding protein 1 in the assembly of hepatitis C virus potentially via their interaction with the 3' untranslated region of the viral genome; Authors - Jie Liu