RPGR Polyclonal antibody, PBS Only

RPGR Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16891-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of mouse eye using 16891-1-AP (same clone as 16891-1-PBS)

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 16891-1-AP (RPGR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 16891-1-AP (same clone as 16891-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16891-1-AP (RPGR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 16891-1-AP (same clone as 16891-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16891-1-AP (RPGR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Y79 using 16891-1-AP (same clone as 16891-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 16891-1-AP (RPGR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 16891-1-AP (same clone as 16891-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16891-1-AP (RPGR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 16891-1-AP (same clone as 16891-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16891-1-AP (RPGR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse eye using 16891-1-AP (same clone as 16891-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 16891-1-AP (RPGR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse eye using 16891-1-AP (same clone as 16891-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 16891-1-AP (RPGR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 16891-1-AP (same clone as 16891-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16891-1-AP (RPGR Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 16891-1-AP (same clone as 16891-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16891-1-AP (RPGR Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 16891-1-AP (same clone as 16891-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16891-1-AP (RPGR Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 16891-1-AP (same clone as 16891-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16891-1-AP (RPGR Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

• 

  IF Staining of hTERT-RPE1 cells using 16891-1-AP (same clone as 16891-1-PBS)

  IF result of anti-RPGR (16891-1-AP) in hTERT-RPE1 cell by Dr. Seongjin Seo. This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

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Product Information

16891-1-PBS targets RPGR in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPGR fusion protein Ag10405
• Full Name: retinitis pigmentosa GTPase regulator
• Calculated Molecular Weight: 113 kDa
• Observed Molecular Weight: 100-105 kDa, 70 kDa
• GenBank Accession Number: BC031624
• Gene Symbol: RPGR
• Gene ID (NCBI): 6103
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

RPGR is a protein with a series of six RCC1-like domains (RLDs), characteristic of the highly conserved guanine nucleotide exchange factors. The encoded protein is found in the Golgi body and interacts with RPGRIP1. This protein localizes to the outer segment of rod photoreceptors and is essential for their viability. Mutations in this gene have been associated with X-linked retinitis pigmentosa (XLRP). Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length natures of only some have been determined. This antibody can recognize all the isoforms of RPGR.