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RPGR Polyclonal antibody

RPGR Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16891-1-AP

Synonyms

XLRP3, X-linked retinitis pigmentosa GTPase regulator, X linked retinitis pigmentosa GTPase regulator, RP3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse eye tissue using RPGR Polyclonal antibody (16891-1-AP)

    WB analysis of mouse eye using 16891-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 16891-1-AP (RPGR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RPGR Polyclonal antibody (16891-1-AP)

    WB analysis using 16891-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16891-1-AP (RPGR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using RPGR Polyclonal antibody (16891-1-AP)

    WB analysis of SH-SY5Y using 16891-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16891-1-AP (RPGR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using RPGR Polyclonal antibody (16891-1-AP)

    WB analysis of mouse liver using 16891-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16891-1-AP (RPGR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using RPGR Polyclonal antibody (16891-1-AP)

    WB analysis of Y79 using 16891-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 16891-1-AP (RPGR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using RPGR Polyclonal antibody (16891-1-AP)

    WB analysis of SH-SY5Y using 16891-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16891-1-AP (RPGR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using RPGR Polyclonal antibody (16891-1-AP)

    IHC staining of mouse eye using 16891-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 16891-1-AP (RPGR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using RPGR Polyclonal antibody (16891-1-AP)

    IHC staining of mouse eye using 16891-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 16891-1-AP (RPGR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using RPGR Polyclonal antibody (16891-1-AP)

    IHC staining of human kidney using 16891-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16891-1-AP (RPGR Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using RPGR Polyclonal antibody (16891-1-AP)

    IHC staining of human kidney using 16891-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16891-1-AP (RPGR Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using RPGR Polyclonal antibody (16891-1-AP)

    IHC staining of human heart using 16891-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16891-1-AP (RPGR Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using RPGR Polyclonal antibody (16891-1-AP)

    IHC staining of human heart using 16891-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16891-1-AP (RPGR Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using RPGR Polyclonal antibody (16891-1-AP)

    IF Staining of hTERT-RPE1 cells using 16891-1-AP

    IF result of anti-RPGR (16891-1-AP) in hTERT-RPE1 cell by Dr. Seongjin Seo.

Tested Applications

Positive WB detected inmouse eye tissue, mouse liver tissue, Y79 cells, SH-SY5Y cells, mouse retina tissue
Positive IHC detected inmouse eye tissue, human heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

16891-1-AP targets RPGR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPGR fusion protein Ag10405
Full Name retinitis pigmentosa GTPase regulator
Calculated Molecular Weight 113 kDa
Observed Molecular Weight 100-105 kDa, 70 kDa
GenBank Accession NumberBC031624
Gene Symbol RPGR
Gene ID (NCBI) 6103
RRIDAB_2181130
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPGR is a protein with a series of six RCC1-like domains (RLDs), characteristic of the highly conserved guanine nucleotide exchange factors. The encoded protein is found in the Golgi body and interacts with RPGRIP1. This protein localizes to the outer segment of rod photoreceptors and is essential for their viability. Mutations in this gene have been associated with X-linked retinitis pigmentosa (XLRP). Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length natures of only some have been determined. This antibody can recognize all the isoforms of RPGR.

Protocols

Product Specific Protocols
WB protocol for RPGR antibody 16891-1-APDownload protocol
IHC protocol for RPGR antibody 16891-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Elife

Multiple ciliary localization signals control INPP5E ciliary targeting

Authors - Dario Cilleros-Rodriguez
View Article
humanWB

J Pers Med

Human iPSC-Derived Retinal Organoids and Retinal Pigment Epithelium for Novel Intronic RPGR Variant Assessment for Therapy Suitability.

Authors - Fidelle Chahine Karam
View Article
humanWB

Cell Rep

RPGR is a guanine nucleotide exchange factor for the small GTPase RAB37 required for retinal function via autophagy regulation

Authors - Ruhong Ying
View Article