ROR2 Monoclonal antibody

ROR2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1A12C5

Cat no : 67906-1-Ig

Synonyms

BDB, BDB1, NTRKR2, ROR2

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Validation Data Gallery

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  WB analysis using 67906-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67906-1-Ig (ROR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis using 67906-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67906-1-Ig (ROR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67906-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67906-1-Ig (ROR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HSC-T6 cells, NIH/3T3 cells, MDA-MB-231 cells, T-47D cells, HeLa cells, K-562 cells, Jurkat cells, 4T1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67906-1-Ig targets ROR2 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ROR2 fusion protein Ag31197
• Full Name: receptor tyrosine kinase-like orphan receptor 2
• Calculated Molecular Weight: 943 aa, 105 kDa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: BC130522
• Gene Symbol: ROR2
• Gene ID (NCBI): 4920
• RRID: AB_2918662
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tyrosine-protein kinase transmembrane receptor ROR2 belongs to the ROR family of receptor tyrosine kinases (RTKs) (PMID: 12815588). ROR2 is a developmentally regulated protein that has significant expression and roles in a wide variety of tissues during early embryonic development. It was established that ROR2 plays a key role in skeletal and neural organogenesis, as well as in midgut development in the mouse, but then becomes repressed in adult tissues. It may act as a receptor for wnt ligand WNT5A which may result in the inhibition of WNT3A-mediated signaling (PMID: 25614331). Mutations in the ROR2 gene are associated with autosomal recessive Robinow syndrome and autosomal dominant brachydactyly type B (PMID: 12815588).

Publications

Species	Application	Title
mouse	WB	J Nanobiotechnology; A novel microglia-targeting strategy based on nanoparticle-mediated delivery of miR-26a-5p for long-lasting analgesia in chronic pain; Authors - Yitian Lu