ROR1 Monoclonal antibody

• Featured Product
• KD/KO Validated

ROR1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1F5D8

Cat no : 66923-1-Ig

Synonyms

dJ537F10.1, NTRKR1, ROR1

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Validation Data Gallery

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  WB analysis using 66923-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66923-1-Ig (ROR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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  WB analysis using 66923-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66923-1-Ig (ROR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 66923-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66923-1-Ig (ROR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66923-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66923-1-Ig (ROR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66923-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66923-1-Ig (ROR1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66923-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66923-1-Ig (ROR1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, NIH/3T3 cells, HepG2 cells, K-562 cells, NCI-H1299 cells, HEK-293 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 2 publications below

Product Information

66923-1-Ig targets ROR1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ROR1 fusion protein Ag14586
• Full Name: receptor tyrosine kinase-like orphan receptor 1
• Calculated Molecular Weight: 937 aa, 104 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC006374
• Gene Symbol: ROR1
• Gene ID (NCBI): 4919
• RRID: AB_2882250
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ROR1 is a member of the RTK family of orphan receptors related to muscle-specific kinase and Trk neurotrophin receptors (PMID: 18546292). ROR1 is primarily expressed by neural crest cells during embryogenesis. High expression of ROR1 is reported in many types of malignancies and is thought to be involved in tumor growth, apoptosis, and epithelial-mesenchymal transition (PMID: 26245996). The human ROR1 gene encodes a 937 amino acid protein with an Ig-like domain, a cysteine-rich domain, a kringle domain, a tyrosine kinase domain, and a proline-rich domain. The calculated molecular weight of ROR1 is 104 kDa, but ROR1 has multiple N-glycosylation sites that generate post-translationally modified ROR1 at 130 kDa (PMID: 24752542).

Publications

Species	Application	Title
human	WB,IHC	Acta Biochim Biophys Sin (Shanghai); SETD8 induces stemness and epithelial-mesenchymal transition of pancreatic cancer cells by regulating ROR1 expression.; Authors - Mengqi Liu ; KD Validated
human	IHC	Int Immunopharmacol; ROR1/STAT3 positive feedback loop facilitates cartilage degeneration in Osteoarthritis through activation of NF-κB signaling pathway; Authors - Wei Huang

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