ROCK2-Specific(C-term) Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

ROCK2-Specific(C-term) Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20248-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HeLa using 20248-1-AP (same clone as 20248-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 20248-1-AP (same clone as 20248-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 20248-1-AP (same clone as 20248-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 20248-1-AP (same clone as 20248-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 20248-1-AP (same clone as 20248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 20248-1-AP (same clone as 20248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 20248-1-AP (same clone as 20248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 20248-1-AP (same clone as 20248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 20248-1-AP (ROCK2-Specific(C-term) antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 20248-1-AP (same clone as 20248-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using ROCK2 antibody 20248-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20248-1-PBS in a different storage buffer formulation.

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Product Information

20248-1-PBS targets ROCK2-Specific(C-term) in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: Rho-associated, coiled-coil containing protein kinase 2
• Calculated Molecular Weight: 161 kDa
• Observed Molecular Weight: 164 kDa
• GenBank Accession Number: NM_004850
• Gene Symbol: ROCK2
• Gene ID (NCBI): 9475
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. It is an isozyme of ROCK1, is a target for the small GTPase Rho. ROCK2 is localized in both cytoplasm and nucleus, and the nucleus-localized ROCK2 can target p300 for phosphorylation to regulate its acetyltransferase activity (PMID:16574662).This antibody is specific to ROCK2.