ROCK1 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

ROCK1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 21850-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 21850-1-AP (same clone as 21850-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21850-1-AP (ROCK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21850-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 21850-1-AP (same clone as 21850-1-PBS)

  WB result of ROCK1 antibody (21850-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROCK1 transfected HeLa cells. This data was developed using the same antibody clone with 21850-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 21850-1-AP (same clone as 21850-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 21850-1-AP (ROCK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21850-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 21850-1-AP (same clone as 21850-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 21850-1-AP (ROCK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21850-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 21850-1-AP (same clone as 21850-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ROCK1 antibody (21850-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21850-1-PBS in a different storage buffer formulation.

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Product Information

21850-1-PBS targets ROCK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ROCK1 fusion protein Ag16317
• Full Name: Rho-associated, coiled-coil containing protein kinase 1
• Calculated Molecular Weight: 1354 aa, 158 kDa
• Observed Molecular Weight: 150-160 kDa
• GenBank Accession Number: BC113114
• Gene Symbol: ROCK1
• Gene ID (NCBI): 6093
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ROCK1, also named as p160ROCK and NY-REN-35, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. ROCK1 phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. ROCK1 is required for centromere positioning and centromere-dependent exit from mitosis. It is necessary for apoptotic membrane blebbing. ROCK1 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein.