RNGTT Recombinant antibody

RNGTT Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240927A10

Cat no : 83974-2-RR

Synonyms

hCAP, GTP--RNA guanylyltransferase, GTP  RNA guanylyltransferase, GTase, CAP1A

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Validation Data Gallery

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  WB analysis using 83974-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83974-2-RR (RNGTT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 83974-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RNGTT Recombinant antibody (83974-2-RR, Clone:240927A10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 83974-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83974-2-RR against Human RNGTT were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, MCF-7 cells, K-562 cells, HEK-293T cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83974-2-RR is RNGTT Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RNGTT fusion protein Ag3105
• Full Name: RNA guanylyltransferase and 5'-phosphatase
• Calculated Molecular Weight: 597 aa, 69 kDa
• Observed Molecular Weight: 62-68 kDa
• GenBank Accession Number: BC019954
• Gene Symbol: RNGTT
• Gene ID (NCBI): 8732
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNGTT(mRNA-capping enzyme) is also named as HCAP1, HCE, CAP1A. In the N-terminal section it belongs to the non-receptor class of the protein-tyrosine phosphatase family and in the C-terminal section it belongs to the eukaryotic GTase family. It is a bifunctional 597-amino acid protein with RNA triphosphatase and RNA guanylyltransferase activities(PMID:9545288). It has 4 isoforms produced by alternative splicing.