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RNF40 Recombinant antibody, PBS Only

RNF40 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241214A1

Cat no : 84058-5-PBS

Synonyms

E3 ubiquitin-protein ligase BRE1B, BRE1-B, BRE1B, BRE1 B, 95 kDa retinoblastoma-associated protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNF40 Recombinant antibody (84058-5-RR)

    WB analysis using 84058-5-RR (same clone as 84058-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84058-5-RR (RNF40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84058-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RNF40 Recombinant antibody (84058-5-RR)

    IF Staining of HeLa using 84058-5-RR (same clone as 84058-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RNF40 antibody (84058-5-RR, Clone: 241214A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84058-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84058-5-RR

    Affinity and Kinetic Characterization of 84058-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84058-5-RR against Human RNF40 were performed. The affinity constant is below 1 pM.

Product Information

The immunogen of 84058-5-PBS is RNF40 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RNF40 fusion protein Ag8047
Full Name ring finger protein 40
Calculated Molecular Weight 114 kDa
Observed Molecular Weight135 kDa
GenBank Accession NumberBC006133
Gene Symbol RNF40
Gene ID (NCBI) 9810
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The DNA damage response (DDR) is emerging as a vast signaling network that temporarily modulates numerous aspects of cellular metabolism in the face of DNA lesions, especially critical ones such as the double-strand break (DSB). The DDR involves extensive dynamics of protein post-translational modifications, most notably phosphorylation and ubiquitylation. A recently identified novel component of the DDR pathway - histone H2B monoubiquitylation - exemplifies this principle. Monoubiquitination of histone H2B is a dynamic post-translational histone modification associated with transcriptional elongation and the DNA damage response [PMID:22021426]. In mammalian cells, H2B monoubiquitylation is driven primarily by an E3 ubiquitin ligase composed of the two RING finger proteins RNF20 and RNF40 [PMID:22031019,21827756].