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RNF40 Recombinant antibody

RNF40 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241214A1

Cat no : 84058-5-RR

Synonyms

E3 ubiquitin-protein ligase BRE1B, BRE1-B, BRE1B, BRE1 B, 95 kDa retinoblastoma-associated protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNF40 Recombinant antibody (84058-5-RR)

    WB analysis using 84058-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84058-5-RR (RNF40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RNF40 Recombinant antibody (84058-5-RR)

    IF Staining of HeLa using 84058-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RNF40 antibody (84058-5-RR, Clone: 241214A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84058-5-RR

    Affinity and Kinetic Characterization of 84058-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84058-5-RR against Human RNF40 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, A375 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84058-5-RR is RNF40 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RNF40 fusion protein Ag8047
Full Name ring finger protein 40
Calculated Molecular Weight 114 kDa
Observed Molecular Weight135 kDa
GenBank Accession NumberBC006133
Gene Symbol RNF40
Gene ID (NCBI) 9810
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The DNA damage response (DDR) is emerging as a vast signaling network that temporarily modulates numerous aspects of cellular metabolism in the face of DNA lesions, especially critical ones such as the double-strand break (DSB). The DDR involves extensive dynamics of protein post-translational modifications, most notably phosphorylation and ubiquitylation. A recently identified novel component of the DDR pathway - histone H2B monoubiquitylation - exemplifies this principle. Monoubiquitination of histone H2B is a dynamic post-translational histone modification associated with transcriptional elongation and the DNA damage response [PMID:22021426]. In mammalian cells, H2B monoubiquitylation is driven primarily by an E3 ubiquitin ligase composed of the two RING finger proteins RNF20 and RNF40 [PMID:22031019,21827756].

Protocols

Product Specific Protocols
WB protocol for RNF40 antibody 84058-5-RRDownload protocol
IF protocol for RNF40 antibody 84058-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols