CoraLite® Plus 750-conjugated RNF31 / HOIP Recombinant antibody

RNF31 / HOIP Recombinant Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230446E9

Cat no : CL750-83137-4

Synonyms

FLJ10111, FLJ23501, HOIL 1 interacting protein, HOIP, ring finger protein 31, RNF31, ZIBRA

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Validation Data Gallery

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  WB analysis using CL750-83137-4

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-83137-4 (RNF31 / HOIP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL750-83137-4 is RNF31 / HOIP Fusion Protein expressed in E. coli.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RNF31 / HOIP fusion protein Ag34505
• Full Name: ring finger protein 31
• Calculated Molecular Weight: 1072 aa, 120 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC017376
• Gene Symbol: RNF31
• Gene ID (NCBI): 55072
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Ring finger protein 31 (RNF31), also named HOIL-1-interacting protein (HOIP), ZIBRA, is an atypical E3 ubiquitin ligase, belongs to ring finger protein (RNF) family. It was first cloned from breast cancer cell line MCF7 and was identified as a classical component in linear ubiquitin assembly complex (LUBAC) to facilitate NFκB signaling transduction. It also plays important regulatory functions in controlling important oncogenic pathways in breast cancer, such as ERα and p53. RNF31 is highly expressed in muscle, heart, and testis. In cells, RNF31 mainly localizes to the cytoplasm. (PMID:27460922)