CoraLite® Plus 488-conjugated RNF2 Recombinant antibody

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  IF Staining of U2OS using CL488-84793-5

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 RNF2 antibody (CL488-84793-5, Clone: 242181G7 ) at dilution of 1:200, CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

RNF2 Recombinant Antibody for IF/ICC

Cat No. CL488-84793-5

Clone No.242181G7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

242181G7, BAP 1, BAP1, DING, E3 ubiquitin-protein ligase RING2

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Tested Applications

• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84793-5 targets RNF2 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RNF2 fusion protein Ag8900
• Full Name: ring finger protein 2
• Calculated Molecular Weight: 336 aa, 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC012583
• Gene Symbol: RNF2
• Gene ID (NCBI): 6045
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q99496
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RNF2 (also known as ding, Ring1B or Ring2) is a member of the Ring finger protein family, which functions as E3 ubiquitin ligase for monoubiquitination of histone H2A at lysine 119 (H2AK119ub). Many studies have demonstrated that overexpressed RNF2 was involved in the pathological progression of multiple cancers and has an impact on their clinical features. For instance, the upregulated expression level of RNF2 is positively correlated with the occurrence and progression of hepatocellular carcinoma, melanoma, prostate cancer, breast cancer, pancreatic cancer, gastric cancer, and bladder urothelial carcinoma, as well as with the radioresistance of lung cancer and chemoresistance of ovarian cancer (PMID: 33400276). The molecular weight of RNF2 is 38 kDa.