RNF151 Polyclonal antibody, PBS Only

RNF151 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 24708-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of rat testis using 24708-1-AP (same clone as 24708-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 24708-1-AP (RNF151 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24708-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 24708-1-AP (same clone as 24708-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 24708-1-AP (RNF151 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24708-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 24708-1-AP (same clone as 24708-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24708-1-AP (RNF151 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24708-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 24708-1-AP (same clone as 24708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24708-1-AP (RNF151 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24708-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 24708-1-AP (same clone as 24708-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24708-1-AP (RNF151 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24708-1-PBS in a different storage buffer formulation.

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Product Information

24708-1-PBS targets RNF151 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNF151 fusion protein Ag16606
• Full Name: ring finger protein 151
• Calculated Molecular Weight: 245 aa, 27 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC113014
• Gene Symbol: RNF151
• Gene ID (NCBI): 146310
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

RNF151 also termed as ring finger protein 151 is a 245 amino acid protein, which contains one Ring-type zinc finger and one TRAF-type zinc finger. RNF151, with, a putative nuclear localization signal (NLS)was exclusively expressed in the mouse testis and developmentally regulated during spermatogenesis. While RNF151 mRNA was present in round spermatids, its protein was expressed in elongating spermatids of the stage VIII-IX seminiferous tubules.