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RNF151 Polyclonal antibody

RNF151 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24708-1-AP

Synonyms

ring finger protein 151, RNF151

Validation Data Gallery

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  • Western Blot (WB) analysis of rat testis tissue using RNF151 Polyclonal antibody (24708-1-AP)

    WB analysis of rat testis using 24708-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 24708-1-AP (RNF151 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using RNF151 Polyclonal antibody (24708-1-AP)

    WB analysis of human testis using 24708-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 24708-1-AP (RNF151 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using RNF151 Polyclonal antibody (24708-1-AP)

    WB analysis of mouse testis using 24708-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 24708-1-AP (RNF151 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RNF151 Polyclonal antibody (24708-1-AP)

    IHC staining of mouse testis using 24708-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24708-1-AP (RNF151 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RNF151 Polyclonal antibody (24708-1-AP)

    IHC staining of mouse testis using 24708-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24708-1-AP (RNF151 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inrat testis tissue, human testis tissue, mouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24708-1-AP is RNF151 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF151 fusion protein Ag16606
Full Name ring finger protein 151
Calculated Molecular Weight 245 aa, 27 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC113014
Gene Symbol RNF151
Gene ID (NCBI) 146310
RRIDAB_2879683
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNF151 also termed as ring finger protein 151 is a 245 amino acid protein, which contains one Ring-type zinc finger and one TRAF-type zinc finger. RNF151, with, a putative nuclear localization signal (NLS)was exclusively expressed in the mouse testis and developmentally regulated during spermatogenesis. While RNF151 mRNA was present in round spermatids, its protein was expressed in elongating spermatids of the stage VIII-IX seminiferous tubules.

Protocols

Product Specific Protocols
WB protocol for RNF151 antibody 24708-1-APDownload protocol
IHC protocol for RNF151 antibody 24708-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols