Filter: AllWBIHCIF/ICC

WB analysis using 22577-1-AP (same clone as 22577-1-PBS)
THP-1 cells were subjected to SDS PAGE followed by western blot with 22577-1-AP (RNASEL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22577-1-PBS in a different storage buffer formulation.
WB analysis of RAW 264.7 using 22577-1-AP (same clone as 22577-1-PBS)
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 22577-1-AP (RNASEL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22577-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 22577-1-AP (same clone as 22577-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22577-1-AP (RNASEL Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22577-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 22577-1-AP (same clone as 22577-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22577-1-AP (RNASEL Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22577-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 22577-1-AP (same clone as 22577-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22577-1-AP (RNASEL Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22577-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 22577-1-AP (same clone as 22577-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22577-1-AP (RNASEL Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22577-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 22577-1-AP (same clone as 22577-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RNASEL antibody (22577-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22577-1-PBS in a different storage buffer formulation.

RNASEL Polyclonal antibody, PBS Only

RNASEL Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 22577-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

22577-1-PBS targets RNASEL in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RNASEL fusion protein Ag17409 Predict reactive species
Full Name	ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent)
Calculated Molecular Weight	741 aa, 84 kDa
Observed Molecular Weight	84 kDa; 73 kDa
GenBank Accession Number	BC115697
Gene Symbol	RNASEL
Gene ID (NCBI)	6041
RRID	AB_2631036
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen Affinity purified
UNIPROT ID	Q05823
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

RNASEL, also named as RNS4, is an endoribonuclease that functions in the interferon (IFN) antiviral response. RNASEL mediated apoptosis is the result of a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. There're some isoforms with calculated MW 84 kDa and 73 kDa.