RNASEL Polyclonal antibody, PBS Only

RNASEL Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22577-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 22577-1-AP (same clone as 22577-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 22577-1-AP (RNASEL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22577-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 22577-1-AP (same clone as 22577-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 22577-1-AP (RNASEL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22577-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 22577-1-AP (same clone as 22577-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22577-1-AP (RNASEL Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22577-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 22577-1-AP (same clone as 22577-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22577-1-AP (RNASEL Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22577-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 22577-1-AP (same clone as 22577-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22577-1-AP (RNASEL Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22577-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 22577-1-AP (same clone as 22577-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22577-1-AP (RNASEL Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22577-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 22577-1-AP (same clone as 22577-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RNASEL antibody (22577-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22577-1-PBS in a different storage buffer formulation.

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Product Information

22577-1-PBS targets RNASEL in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNASEL fusion protein Ag17409
• Full Name: ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent)
• Calculated Molecular Weight: 741 aa, 84 kDa
• Observed Molecular Weight: 84 kDa; 73 kDa
• GenBank Accession Number: BC115697
• Gene Symbol: RNASEL
• Gene ID (NCBI): 6041
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

RNASEL, also named as RNS4, is an endoribonuclease that functions in the interferon (IFN) antiviral response. RNASEL mediated apoptosis is the result of a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. There're some isoforms with calculated MW 84 kDa and 73 kDa.