RNASEH1 Monoclonal antibody, PBS Only

• 

  WB analysis using 68787-3-Ig (same clone as 68787-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68787-3-Ig (RNASEH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68787-3-PBS in a different storage buffer formulation.

• 

  WB analysis using 68787-3-Ig (same clone as 68787-3-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 68787-3-Ig (RNASEH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68787-3-PBS in a different storage buffer formulation.

RNASEH1 Monoclonal Antibody for WB, Indirect ELISA

Cat No. 68787-3-PBS

Clone No.2C8D3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

RNH1, RNASEH 1, RNase H1, EC:3.1.26.4, 2C8D3

---

Product Information

68787-3-PBS targets RNASEH1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RNASEH1 fusion protein Ag33948
• Full Name: ribonuclease H1
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC002973
• Gene Symbol: RNASEH1
• Gene ID (NCBI): 246243
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O60930
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Ribonucleases H are enzymes that cleave the RNA of RNA/DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. Eukaryotic RNases H are larger and more complex than their prokaryotic counterparts [PMID:12667461]. Eukaryotic RNase H1 has acquired a Hybrid Binding Domain that confers processivity and affinity for the substrate. Because of its essential role and its similarities in structure and enzymatic mechanism to the RNase H of HIV reverse transcriptase, RNASEH1 has been implicated in mitochondrial DNA replication during mouse development [PMID:19228196].