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RNASEH1 Monoclonal antibody

RNASEH1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2C8D3

Cat no : 68787-3-Ig

Synonyms

RNH1, RNASEH 1, RNase H1, EC:3.1.26.4, 2C8D3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNASEH1 Monoclonal antibody (68787-3-Ig)

    WB analysis using 68787-3-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68787-3-Ig (RNASEH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RNASEH1 Monoclonal antibody (68787-3-Ig)

    WB analysis using 68787-3-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 68787-3-Ig (RNASEH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inSK-N-SH cells, 4T1 cells, A549 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68787-3-Ig targets RNASEH1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RNASEH1 fusion protein Ag33948
Full Name ribonuclease H1
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC002973
Gene Symbol RNASEH1
Gene ID (NCBI) 246243
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribonucleases H are enzymes that cleave the RNA of RNA/DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. Eukaryotic RNases H are larger and more complex than their prokaryotic counterparts [PMID:12667461]. Eukaryotic RNase H1 has acquired a Hybrid Binding Domain that confers processivity and affinity for the substrate. Because of its essential role and its similarities in structure and enzymatic mechanism to the RNase H of HIV reverse transcriptase, RNASEH1 has been implicated in mitochondrial DNA replication during mouse development [PMID:19228196].

Protocols

Product Specific Protocols
WB protocol for RNASEH1 antibody 68787-3-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols