RING1 Polyclonal antibody, PBS Only

RING1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15037-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 15037-1-AP (same clone as 15037-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15037-1-AP (RING1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15037-1-PBS in a different storage buffer formulation.

• 

  WB analysis of BxPC-3 using 15037-1-AP (same clone as 15037-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15037-1-AP (RING1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15037-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 15037-1-AP (same clone as 15037-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15037-1-AP (RING1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15037-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 15037-1-AP (same clone as 15037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15037-1-AP (RING1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15037-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 15037-1-AP (same clone as 15037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15037-1-AP (RING1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15037-1-PBS in a different storage buffer formulation.

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Product Information

15037-1-PBS targets RING1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RING1 fusion protein Ag6763
• Full Name: ring finger protein 1
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC002922
• Gene Symbol: RING1
• Gene ID (NCBI): 6015
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Ring1, also named as Ring1a, constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It's an essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.