CoraLite® Plus 488-conjugated RIG-1/DDX58 Monoclonal antibody

RIG-1/DDX58 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B10F5

Cat no : CL488-67556

Synonyms

DDX58, DEAD box protein 58, DKFZp434J1111, DKFZp686N19181, FLJ13599, RIG 1, RIG I, RIG-1/DDX58, RIG-I

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-67556

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488-conjugated RIG-1/DDX58 antibody (CL488-67556, Clone: 3B10F5 ) at dilution of 1:100.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67556 targets RIG-1/DDX58 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RIG-1/DDX58 fusion protein Ag18591
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
• Calculated Molecular Weight: 925 aa, 106 kDa
• Observed Molecular Weight: 101/106 kDa
• GenBank Accession Number: BC132786
• Gene Symbol: RIG-1/DDX58
• Gene ID (NCBI): 23586
• RRID: AB_2919499
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DDX58, also named as RIG-1, belongs to the helicase family. It is involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). It is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV. The antibody is specific to DDX58,which has two isoform with the MW of 106 and 101 kDa.