CoraLite® Plus 488-conjugated RIAM,APBB1IP Monoclonal antibody

RIAM,APBB1IP Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F8A2

Cat no : CL488-67143

Synonyms

APBB1 interacting protein 1, APBB1IP, APBB1IP,RIAM, INAG1, PREL 1, PREL1, Proline rich EVH1 ligand 1, Proline rich protein 73, RARP 1, RARP1, RIAM, RIAM,APBB1IP

---

Validation Data Gallery

• 

  IF Staining of Jurkat using CL488-67143

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 488 RIAM,APBB1IP antibody (CL488-67143, Clone: 2F8A2 ) at dilution of 1:200.

---

Tested Applications

• Positive IF/ICC detected in: Jurkat cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67143 targets RIAM,APBB1IP in IF/ICC applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RIAM,APBB1IP fusion protein Ag4602
• Full Name: amyloid beta (A4) precursor protein-binding, family B, member 1 interacting protein
• Calculated Molecular Weight: 666 aa, 73 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC035636
• Gene Symbol: RIAM
• Gene ID (NCBI): 54518
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

APBB1IP gene encodes a Rap1-GTP-interacting adapter molecule (RIAM) which is an important protein in Rap1-mediated integrin activation and adhesion. Rap1 is a small GTPase frequently activated in tumors such as melanoma and prostate cancer. RIAM is widely expressed with high expression in thymus, spleen, lymph node, bone marrow and peripheral leukocytes. RIAM may function in the signal transduction from Ras activation to actin cytoskeletal remodeling. The observed molecular weight of RIAM is about 100 kDa.