RHEB Monoclonal antibody, PBS Only

RHEB Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F9H8

Cat no : 66714-1-PBS

Synonyms

RHEB2, Ras homolog enriched in brain, GTP-binding protein Rheb, GTP binding protein Rheb, EC:3.6.5.-

---

Validation Data Gallery

• 

  WB analysis of fetal human brain using 66714-1-Ig (same clone as 66714-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66714-1-Ig (RHEB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66714-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66714-1-Ig (same clone as 66714-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66714-1-Ig (RHEB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66714-1-PBS in a different storage buffer formulation.

• 

  WB analysis of DU 145 using 66714-1-Ig (same clone as 66714-1-PBS)

  DU 145 cells were subjected to SDS PAGE followed by western blot with 66714-1-Ig (RHEB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66714-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 66714-1-Ig (same clone as 66714-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66714-1-Ig (RHEB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66714-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 66714-1-Ig (same clone as 66714-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66714-1-Ig (RHEB antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66714-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human pancreas cancer using 66714-1-Ig (same clone as 66714-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using RHEB antibody (66714-1-Ig, Clone: 2F9H8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66714-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human pancreas cancer using 66714-1-Ig (same clone as 66714-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using RHEB antibody (66714-1-Ig, Clone: 2F9H8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66714-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse pancreas using 66714-1-Ig (same clone as 66714-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using RHEB antibody (66714-1-Ig, Clone: 2F9H8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66714-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse pancreas using 66714-1-Ig (same clone as 66714-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using RHEB antibody (66714-1-Ig, Clone: 2F9H8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66714-1-PBS in a different storage buffer formulation.

---

Product Information

66714-1-PBS targets RHEB in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RHEB fusion protein Ag8887
• Full Name: Ras homolog enriched in brain
• Calculated Molecular Weight: 184 aa, 20 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC016155
• Gene Symbol: RHEB
• Gene ID (NCBI): 6009
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Rheb is a member of the Ras superfamily small GTPases that plays important roles in the regulation of protein synthesis and growth in response to nutrient and growth factors. It is well known to bind and activate mTOR, a serine/threonine kinase which augments protein synthesis. Rheb has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity.