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RHEB Monoclonal antibody

RHEB Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2F9H8

Cat no : 66714-1-Ig

Synonyms

RHEB2, Ras homolog enriched in brain, GTP-binding protein Rheb, GTP binding protein Rheb, EC:3.6.5.-

Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using RHEB Monoclonal antibody (66714-1-Ig)

    WB analysis of fetal human brain using 66714-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66714-1-Ig (RHEB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using RHEB Monoclonal antibody (66714-1-Ig)

    WB analysis of mouse brain using 66714-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66714-1-Ig (RHEB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RHEB Monoclonal antibody (66714-1-Ig)

    WB analysis of HeLa using 66714-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66714-1-Ig (RHEB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of DU 145 cells using RHEB Monoclonal antibody (66714-1-Ig)

    WB analysis of DU 145 using 66714-1-Ig

    DU 145 cells were subjected to SDS PAGE followed by western blot with 66714-1-Ig (RHEB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using RHEB Monoclonal antibody (66714-1-Ig)

    IHC staining of human skin cancer using 66714-1-Ig

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66714-1-Ig (RHEB antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human pancreas cancer tissue using RHEB Monoclonal antibody (66714-1-Ig)

    IF Staining of human pancreas cancer using 66714-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using RHEB antibody (66714-1-Ig, Clone: 2F9H8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using RHEB Monoclonal antibody (66714-1-Ig)

    IF Staining of mouse pancreas using 66714-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using RHEB antibody (66714-1-Ig, Clone: 2F9H8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human pancreas cancer tissue using RHEB Monoclonal antibody (66714-1-Ig)

    IF Staining of human pancreas cancer using 66714-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using RHEB antibody (66714-1-Ig, Clone: 2F9H8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using RHEB Monoclonal antibody (66714-1-Ig)

    IF Staining of mouse pancreas using 66714-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using RHEB antibody (66714-1-Ig, Clone: 2F9H8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected infetal human brain tissue, HeLa cells, DU 145 cells, mouse brain tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman pancreas cancer tissue, mouse pancreas tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66714-1-Ig targets RHEB in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RHEB fusion protein Ag8887
Full Name Ras homolog enriched in brain
Calculated Molecular Weight 184 aa, 20 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC016155
Gene Symbol RHEB
Gene ID (NCBI) 6009
RRIDAB_2882065
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rheb is a member of the Ras superfamily small GTPases that plays important roles in the regulation of protein synthesis and growth in response to nutrient and growth factors. It is well known to bind and activate mTOR, a serine/threonine kinase which augments protein synthesis. Rheb has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity.

Protocols

Product Specific Protocols
WB protocol for RHEB antibody 66714-1-IgDownload protocol
IHC protocol for RHEB antibody 66714-1-IgDownload protocol
IF protocol for RHEB antibody 66714-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols