RFC5 Polyclonal antibody

RFC5 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF

Conjugate

Unconjugated

Cat no : 28671-1-AP

Synonyms

A1 36 kDa subunit, Activator 1 36 kDa subunit, Activator 1 subunit 5, Replication factor C subunit 5, RF C 36 kDa subunit, RFC36, RFC5

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Validation Data Gallery

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  WB analysis using 28671-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28671-1-AP (RFC5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 28671-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RFC5 antibody (28671-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 28671-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RFC5 antibody (28671-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HCT 116 cells, U-251 cells, HEK-293T cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28671-1-AP targets RFC5 in WB, IF applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RFC5 fusion protein Ag29236
• Full Name: replication factor C (activator 1) 5, 36.5kDa
• Calculated Molecular Weight: 36 aa, 5 kDa
• GenBank Accession Number: BC001866
• Gene Symbol: RFC5
• Gene ID (NCBI): 5985
• RRID: AB_2918189
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Replication factor C subunit 5 (RFC5), also known as activator 1, can recruit proliferating cell nuclear antigen(PCNA) onto the DNA polymerase S-phase checkpoint complex. In eukaryotes, RFC5 is involved in repairing mismatches, DNA double helix damage, nucleotide excision, and regulating the cell cycle(PMID: 30210909). RFC5 is significantly upregulated in cancer tissues or cells, and its expression is elevated with the cancer progression(PMID: 30214556).