RENALASE Monoclonal antibody, PBS Only

RENALASE Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A12F5

Cat no : 60128-1-PBS

Synonyms

C10orf59, 3A12F5, Monoamine oxidase C, MAO-C, MAO C

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Validation Data Gallery

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  WB analysis using 60128-1-Ig (same clone as 60128-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60128-1-Ig (RENALASE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60128-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60128-1-Ig (same clone as 60128-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60128-1-Ig (RENALASE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60128-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MG-63 using 60128-1-Ig (same clone as 60128-1-PBS)

  MG-63 cells were subjected to SDS PAGE followed by western blot with 60128-1-Ig (RENALASE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60128-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60128-1-Ig (same clone as 60128-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 60128-1-Ig (RENALASE Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60128-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60128-1-Ig (same clone as 60128-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 60128-1-Ig (RENALASE Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60128-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 60128-1-Ig (same clone as 60128-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 60128-1-Ig (RENALASE Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60128-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 60128-1-Ig (same clone as 60128-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 60128-1-Ig (RENALASE Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60128-1-PBS in a different storage buffer formulation.

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Product Information

60128-1-PBS targets RENALASE in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RENALASE fusion protein Ag13061
• Full Name: chromosome 10 open reading frame 59
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC005364
• Gene Symbol: RENALASE
• Gene ID (NCBI): 55328
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

RNLS, also named as Renalase, C10orf59 and MAO-C, belongs to the renalase family. It is probable FAD-dependent amine oxidase secreted by the kidney, which circulates in blood and modulates cardiac function and systemic blood pressure. RNLS degrades catecholamines such as dopamine, norepinephrine and epinephrine in vitro. It lowers blood pressure in vivo by decreasing cardiac contractility and heart rate and preventing a compensatory increase in peripheral vascular tone, suggesting a causal link to the increased plasma catecholamine and heightened cardiovascular risk. High concentrations of catecholamines activate plasma renalase and promotes its secretion and synthesis. RNLS has physiologically relevant catecholamine-oxidizing activity. (PMID:15841207 ) This antibody is specific to RNLS.