RENALASE Monoclonal antibody

RENALASE Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3A12F5

Cat no : 60128-1-Ig

Synonyms

C10orf59, 3A12F5, Monoamine oxidase C, MAO-C, MAO C



Tested Applications

Positive WB detected inOS-RC-2 cells, A431 cells, MG-63 cells, ACHN cells, Caki-1 cells
Positive IHC detected inhuman kidney tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

60128-1-Ig targets RENALASE in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RENALASE fusion protein Ag13061
Full Name chromosome 10 open reading frame 59
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC005364
Gene Symbol RENALASE
Gene ID (NCBI) 55328
RRIDAB_2034903
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNLS, also named as Renalase, C10orf59 and MAO-C, belongs to the renalase family. It is probable FAD-dependent amine oxidase secreted by the kidney, which circulates in blood and modulates cardiac function and systemic blood pressure. RNLS degrades catecholamines such as dopamine, norepinephrine and epinephrine in vitro. It lowers blood pressure in vivo by decreasing cardiac contractility and heart rate and preventing a compensatory increase in peripheral vascular tone, suggesting a causal link to the increased plasma catecholamine and heightened cardiovascular risk. High concentrations of catecholamines activate plasma renalase and promotes its secretion and synthesis. RNLS has physiologically relevant catecholamine-oxidizing activity. (PMID:15841207 ) This antibody is specific to RNLS.

Protocols

Product Specific Protocols
WB protocol for RENALASE antibody 60128-1-IgDownload protocol
IHC protocol for RENALASE antibody 60128-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nephrol Dial Transplant

Renalase in hypertension and kidney disease.

Authors - Desir Gary V GV
humanWB

J Am Soc Hypertens

Human renalase: a review of its biology, function, and implications for hypertension.

Authors - Desir Gary V GV
mouseWB

bioRxiv

Renalase inhibition regulates β cell metabolism to defend against acute and chronic stress

Authors - Tara MacDonald