RELB Recombinant antibody

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  WB analysis using 83305-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83305-6-RR (RELB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of Raji using 83305-6-RR

  1x10^6 Raji cells were intracellularly stained with 0.25 ug RELB Recombinant antibody (83305-6-RR, Clone:242336G4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83305-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83305-6-RR against Human RELB were performed. The affinity constant is below 1 pM.

RELB Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 83305-6-RR

Clone No.242336G4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

Transcription factor RelB, I-Rel, 242336G4

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Tested Applications

• Positive WB detected in: Raji cells, Daudi cells, MDA-MB-231 cells
• Positive FC (Intra) detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83305-6-RR targets RELB in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RELB fusion protein Ag13824
• Full Name: v-rel reticuloendotheliosis viral oncogene homolog B
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 62-65 kDa
• GenBank Accession Number: BC028013
• Gene Symbol: RELB
• Gene ID (NCBI): 5971
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q01201
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RELB, also named as Transcription factor RelB, is a 579 amino acid protein, which contains one RHD domain. RELB localizes in the nucleus and cytoplasm. RELB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis.