RELB Polyclonal antibody

RELB Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 25027-1-AP

Synonyms

I REL, IREL, RELB, Transcription factor RelB



Tested Applications

Positive WB detected inRaji cells, Ramos cells, mouse thymus tissue
Positive IP detected inRaji cells
Positive IHC detected inhuman colon cancer tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

25027-1-AP targets RELB in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RELB fusion protein Ag13824
Full Name v-rel reticuloendotheliosis viral oncogene homolog B
Calculated molecular weight 62 kDa
Observed molecular weight 62-65 kDa
GenBank accession numberBC028013
Gene symbol RELB
Gene ID (NCBI) 5971
RRIDAB_2879856
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RELB, also named as Transcription factor RelB, is a 579 amino acid protein, which contains one RHD domain. RELB localizes in the nucleus and cytoplasm. RELB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis.

Protocols

Product Specific Protocols
WB protocol for RELB antibody 25027-1-APDownload protocol
IHC protocol for RELB antibody 25027-1-APDownload protocol
IF protocol for RELB antibody 25027-1-APDownload protocol
IP protocol for RELB antibody 25027-1-APDownload protocol
FC protocol for RELB antibody 25027-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IHC

Nat Genet

Exome sequencing identifies somatic mutations of DDX3X in natural killer/T-cell lymphoma.

Authors - Lu Jiang
humanIF

Nucleic Acids Res

p38-mediated FOXN3 phosphorylation modulates lung inflammation and injury through the NF-κB signaling pathway

Authors - Xinxing Zhu
humanWB

Cell Death Dis

Identification of 11(13)-dehydroivaxillin as a potent therapeutic agent against non-Hodgkin's lymphoma.

Authors - Xinhua Xiao
humanWB

J Clin Endocrinol Metab

Circulating Myeloid-derived Suppressor Cells Facilitate Invasion of Thyroid Cancer Cells by Repressing miR-486-3p.

Authors - Li Chen
humanFC

Front Oncol

NF-κB fingerprinting reveals heterogeneous NF-κB composition in diffuse large B-cell lymphoma

Authors - Eleanor Jayawant
mouse,humanWB

Antioxidants (Basel)

Chlorogenic Acid Alleviated AFB1-Induced Hepatotoxicity by Regulating Mitochondrial Function, Activating Nrf2/HO-1, and Inhibiting Noncanonical NF-κB Signaling Pathway

Authors - Qianqian Wang