RELB Polyclonal antibody

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  WB analysis using 25027-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25027-1-AP (RELB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 25027-1-AP

  IP result of anti-RELB (IP:25027-1-AP, 4ug; Detection:25027-1-AP 1:3000) with Raji cells lysate 1600 ug.

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  IHC staining of human colon cancer using 25027-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25027-1-AP (RELB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 25027-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25027-1-AP (RELB Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 25027-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25027-1-AP (RELB Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 25027-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25027-1-AP (RELB Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lymphoma using 25027-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25027-1-AP (RELB Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 25027-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25027-1-AP (RELB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 25027-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25027-1-AP (RELB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 25027-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25027-1-AP (RELB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 25027-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25027-1-AP (RELB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 25027-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RELB antibody (25027-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

RELB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 25027-1-AP

Publications(13)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

I-Rel, Transcription factor RelB

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Tested Applications

• Positive WB detected in: Daudi cells, Raji cells, Ramos cells
• Positive IP detected in: Raji cells
• Positive IHC detected in: human colon cancer tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

25027-1-AP targets RELB in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13824

  Product name: Recombinant human RELB protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 264-579 aa of BC028013

  Sequence: NVVRICFQASYRDQQGQMRRMDPVLSEPVYDKKSTNTSELRICRINKESGPCTGGEELYLLCDKVQKEDISVVFSRASWEGRADFSQADVHRQIAIVFKTPPYEDLEIVEPVTVNVFLQRLTDGVCSEPLPFTYLPRDHDSYGVDKKRKRGMPDVLGELNSSDPHGIESKRRKKKPAILDHFLPNHGSGPFLPPSALLPDPDFFSGTVSLPGLEPPGGPDLLDDGFAYDPTAPTLFTMLDLLPPAPPHASAVVCSGGAGAVVGETPGPEPLTLDSYQAPGPGDGGTASLVGSNMFPNHYREAAFGGGLLSPGPEAT

• Full Name: v-rel reticuloendotheliosis viral oncogene homolog B
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 62-65 kDa
• GenBank Accession Number: BC028013
• Gene Symbol: RELB
• Gene ID (NCBI): 5971
• RRID: AB_2879856
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q01201
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RELB, also named as Transcription factor RelB, is a 579 amino acid protein, which contains one RHD domain. RELB localizes in the nucleus and cytoplasm. RELB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Nat Genet; Exome sequencing identifies somatic mutations of DDX3X in natural killer/T-cell lymphoma.; Authors - Lu Jiang
human	IF	Nucleic Acids Res; p38-mediated FOXN3 phosphorylation modulates lung inflammation and injury through the NF-κB signaling pathway; Authors - Xinxing Zhu
human	WB	Biochim Biophys Acta Mol Basis Dis; LRRC8A as a central mediator promotes colon cancer metastasis by regulating PIP5K1B/PIP2 pathway; Authors - Haifeng Zhang
human	WB	Cell Death Dis; Identification of 11(13)-dehydroivaxillin as a potent therapeutic agent against non-Hodgkin's lymphoma.; Authors - Xinhua Xiao
	WB	J Mol Biol; Pim1 is critical in T-cell-independent B-cell response and MAPK activation in B cells; Authors - Dongya Cui
human	WB	J Clin Endocrinol Metab; Circulating Myeloid-derived Suppressor Cells Facilitate Invasion of Thyroid Cancer Cells by Repressing miR-486-3p.; Authors - Li Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Laura (Verified Customer) (04-18-2025)

Antibody seemed to work well - will start using for my in vivo experiments

• Applications: Immunohistochemistry, Immunofluorescence, Flow Cytometry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Bladder epithelium

RELB Antibody Immunohistochemistry,Immunofluorescence,Flow Cytometry validation (1:100 dilution) in Bladder epithelium (Cat no:25027-1-AP)