CoraLite® Plus 488-conjugated RDBP Recombinant antibody

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  IF Staining of HeLa using CL488-85945

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 RDBP antibody (CL488-85945, Clone: 250420G2 ) at dilution of 1:200, CL594-Phalloidin (red).

RDBP Recombinant Antibody for IF/ICC

Cat No. CL488-85945

Clone No.250420G2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

NELFE, NELF E, NELF-E, RD, RNA-binding protein RD

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-85945 targets RDBP in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RDBP fusion protein Ag1186
• Full Name: RD RNA binding protein
• Calculated Molecular Weight: 37 kDa, 43 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC011600
• Gene Symbol: NELFE
• Gene ID (NCBI): 7936
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P18615
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RDBP, also named NELF-E and RD, belongs to the RRM NELF-E family. It is an essential component of the NELF complex which is a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, a complex acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. RDBP was recently found implicated in RNA polymerase II pausing by association with Hsp90. Catalog# 10705-1-AP is a widely used rabbit polyclonal antibody obtained by immunizing animals with full-length RDBP of human origin.